Abstract:
Although the real structure is not known, however since its first discovery in 2004, carbon dots (CNDs), a new class of carbogenic nanomaterials has paid lots of attention to the nanomaterials research community. This is mainly due to their easy and low cost synthesis
method, easy cell permeability, high photo stability, brightness and more over non toxicity for cellular bioimaging. Both the top down and bottom up approach have been used for the successful synthesis of CNDs. The present thesis deals with the bottom up approach for easy and fast microwave assisted synthesis of doped CNDs from different precursor molecules, its surface passivation or functionalization and its potential applications on the ultrasensitive and highly selective toxic heavy metal ions and small biomolecules detection, even in live cell. Several standard and advanced techniques were used for material characterization. For the analytical observation on sensing applications mainly steady state and time resolved fluorescence were used.
Confocal microscopy along with several laser excitation sources were used for the live cell imaging and bio application of the CNDs.